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Phenotypic Stability of Mature Dendritic Cells Tuned by TLR or CD40 to Control the Efficiency of Cytotoxic T Cell Priming
Author(s) -
Nakamura Ichiro,
Kajino Kiichi,
Bamba Hiromichi,
Itoh Fumito,
Takikita Mikiko,
Ogasawara Kazumasa
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03508.x
Subject(s) - ctl* , biology , cd40 , priming (agriculture) , stimulation , cytotoxic t cell , immunology , dendritic cell , antigen , microbiology and biotechnology , major histocompatibility complex , mhc class ii , mhc class i , antigen presentation , immune system , t cell , in vitro , cd8 , neuroscience , genetics , germination , botany
It is generally accepted that after stimulation immature DCs turn into mature DCs, which present exogenous antigens together with their MHC class I molecules and then activate the antigen‐specific CTLs. Although both TLR and CD40 stimulation appeared to provide the same effects on DC maturation, CD40‐dependent CTL activation is much more potent than CTL activation through LPS stimulation. Despite their different outcomes, the factors that lead mature DCs to different functions remain largely undefined. In this study, we defined the transient maturation and subsequent deactivation of DCs by TLR stimuli, including those by LPS and CpG‐ODN. In contrast, CD40 stimulation induced stable mature DCs that elicited sufficient CTL proliferation. The deactivated DCs, which we defined as “expired DCs,” were phenotypically similar to immature DCs, except for their phenotype stability, MHC class I expression level and IL‐10 production. Moreover, the functions of expired DCs were comparable to those of immature DCs in terms of CTL induction and tolerogenicity. These results may provide an explanation for the role of CD40 stimulation in antigen‐specific CTL induction.