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Human Herpesvirus 6 Immediate‐Early 2 Protein Interacts with Heterogeneous Ribonucleoprotein K and Casein Kinase 2
Author(s) -
Shimada Kazuya,
Kondo Kazuhiro,
Yamanishi Koichi
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03507.x
Subject(s) - ribonucleoprotein , biology , immunoprecipitation , casein kinase 2 , heterogeneous nuclear ribonucleoprotein , heterogeneous ribonucleoprotein particle , protein subunit , colocalization , transcription (linguistics) , microbiology and biotechnology , rna binding protein , rna , casein kinase 1 , virology , kinase , protein kinase a , genetics , gene , cyclin dependent kinase 2 , linguistics , philosophy
Human herpesvirus 6 (HHV‐6) immediate‐early (IE) 2 protein (IE2) may play important but incompletely defined roles during infection. We used yeast two‐hybrid screening to detect proteins interacting with HHV‐6 IE2, and found heterogeneous nuclear ribonucleoprotein K (hnRNP K) and the beta subunit of casein kinase 2 (CK2β) specifically interacted with HHV‐6 IE2. The interactions were confirmed by GST pull‐down assay, coimmunoprecipitation, and colocalization studies. These findings indicate that the HHV‐6 IE2 protein interacts with hnRNP K and CK2, and these interactions may affect viral and cellular RNA transcription and translation in viral replication.