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Effects of Artificial Codon Changes in the Movement Protein Gene on Adaptation of a Hybrid Bromovirus to Cowpea
Author(s) -
Sasaki Nobumitsu,
Kaido Masanori,
Okuno Tetsuro,
Mise Kazuyuki
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03499.x
Subject(s) - biology , brome mosaic virus , movement protein , gene , mutant , cowpea mosaic virus , virus , adaptation (eye) , genetics , virology , stop codon , coat protein , rna , rna dependent rna polymerase , neuroscience
A hybrid Cowpea chlorotic mottle virus (CCMV) with the movement protein (MP) gene replaced with that of the closely related Brome mosaic virus cannot infect cowpea systemically. Twenty‐nine spontaneous mutants from the hybrid CCMV capable of systemic infection in cowpea appeared through biased codon changes that resulted in Lys or Arg at five specific positions in the MP gene. In this study, we report that systemic infection of cowpea with the hybrid CCMV can be achieved by artificial codon changes that do not result in Lys or Arg. We discuss mechanisms that restrict the occurrence of cowpeaadapted mutants in nature.