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Comparison of Immunological Responses to the Various Types of Circumsporozoite Proteins of Plasmodium vivax in Malaria Patients of Korea
Author(s) -
Suh In Bum,
Lee KeeHoyung,
Kim Young Ree,
Woo Sang Kyu,
Kang Heui Yeon,
Won Yoo Duck,
An Seong Soo A.,
Cho Min,
Cho Duck,
Lim Chae Seung
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03497.x
Subject(s) - circumsporozoite protein , plasmodium vivax , malaria , biology , plasmodium (life cycle) , virology , vivax malaria , plasmodium falciparum , immunology , immune system , parasite hosting , world wide web , computer science
We compared the seroreactivities against four synthetic peptide antigens (VK210, VK247, Korean type 1, and type 2) and a full length recombinant circumsporozoite protein (CSP) antigen of Plasmodium vivax ( P. vivax ) in samples of sixty‐three tertian malaria patients in Korea. Among the various CSP antigens, the full‐length recombinant CSP showed the highest reactivity in malaria‐exposed groups (85.7%, 54/63). No significant difference was found in the percentage of malaria patients with antibodies among four peptides examined, except a full‐length recombinant CSP. Absorbance values from the peptide‐based ELISA showed high correlations ( r >0.9, P <0.05) at significant values. Five sera without the immunoaffinity against peptides were reactive towards the full‐length recombinant CSP in ELISA. Sera, which were not reactive to a full length recombinant CSP antigen, were not recognized by any of peptide‐based ELISA. These data suggested that peptide structures included in Korean isolates, GNGAGGQAA, and VK247 peptides had immune reactivity and recognition epitopes. Among the antigens, GNGAGGQAA was less recognized by patients exposed to Korean strains of P. vivax in comparison to the VK210 structures.