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Homologous and Heterologous Antibody Responses to Lipopolysaccharide after Enterohemorrhagic Escherichia coli Infection
Author(s) -
Tsutsumi Reiko,
Ichinohe Naoko,
Shimooki Osamu,
Obata Fumiko,
Takahashi Kiomi,
Inada Katsuya,
Sasaki Mika,
Sato Shigehiro,
Chida Shoichi
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03484.x
Subject(s) - serology , escherichia coli , biology , serotype , antibody , heterologous , lipopolysaccharide , microbiology and biotechnology , homologous chromosome , virology , outbreak , escherichia coli infection , immunology , gene , genetics
To evaluate antibody responses against lipopolysaccharide (LPS: O157, O26, and O111) in enterohemorrhagic Escherichia coli (EHEC) infection, sera of 24 schoolchildren associated with the Morioka outbreak in 1997 and of 74 sporadic patients suspected of having EHEC infection were examined. Using a positive standard serum, quantitative evaluation of LPS antibodies by an enzyme‐linked immunosorbent assay (ELISA) was established. High levels of specific IgM and IgA antibodies against homologous E. coli LPS were present in the acute period and are characteristic of EHEC. This could be used for the serological diagnosis of EHEC infection, except for early infants and the elderly. In addition to the specific homologous response, multiple antibody responses against different serotypes other than those isolated were demonstrated in many cases by qualitative analysis using Western blotting.