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Effects of Tacrolimus (FK506) on Encephalomyocarditic Virus‐Induced Diabetes in Mice
Author(s) -
Yama Sachiko,
Nishioka Wakako,
Hirokami Yumiko,
Setoguchi Ruka,
Takeyama Natsumi,
Saeki Keiichi,
Matsumoto Yoshitsugu,
Hayashi Toshiharu,
Doi Kunio,
Onodera Takashi
Publication year - 2004
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2004.tb03482.x
Subject(s) - tacrolimus , insulitis , virus , diabetes mellitus , biology , islet , saline , immunology , ratón , virology , medicine , endocrinology , transplantation
Abstract The effects of tacrolimus on insulin‐dependent diabetes mellitus (IDDM) induced by the D‐variant of encephalomyocarditis virus (D‐EMCV) have been investigated. Male BALB/c mice were treated with tacrolimus before viral inoculation, and then were inoculated with 10 plaque forming units (PFU) of D‐EMCV. The mice continued to be treated with tacrolimus until the animals were sacrificed. D‐EMCV‐infected mice, which were treated with saline as controls, showed abnormal glucose tolerance test (GTT) values, whereas all infected mice with tacrolimus pretreatment were normal on 7 days‐post inoculation (DPI). Histological observations revealed that non‐treated tacrolimus D‐EMCV‐infected mice and which developed diabetes showed severe insulitis in their islets of Langerhans. On the other hand, D‐EMCV‐infected mice treated with tacrolimus were normal. In D‐EMCV‐infected mice, viruses in the pancreata were detected at the same level regardless of treatment with tacrolimus or saline. Expressions of TNF‐α and IFN‐γ mRNA in spleens of tacrolimus‐treated D‐EMCV‐infected mice were lower than that of non‐treated tacrolimus D‐EMCV‐infected mice on 7 DPI. The results suggest that tacrolimus suppresses expressions of TNF‐α and IFN‐γ mRNAs to prevent the onset of D‐EMCV‐induced IDDM.

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