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Molecular Cloning and Characterization of the HmrM Multidrug Efflux Pump from Haemophilus influenzae Rd
Author(s) -
Xu XingJue,
Su XianZhong,
Morita Yuji,
Kuroda Teruo,
Mizushima Tohru,
Tsuchiya Tomofusa
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb03467.x
Subject(s) - efflux , ethidium bromide , biology , norfloxacin , escherichia coli , microbiology and biotechnology , acriflavine , plasmid , multiple drug resistance , molecular cloning , antiporter , drug resistance , gene , dna , biochemistry , peptide sequence , antibiotics , ciprofloxacin , membrane
We cloned a gene responsible for norfloxacin resistance from the chromosomal DNA of Haemophilus influenzae Rd, and designated the gene as hmrM . HmrM showed sequence similarity with NorM of Vibrio parahaemolyticus and YdhE of Escherichia coli and others that belong to the MATE family multidrug efflux pumps. The recombinant plasmid carrying the hmrM gene conferred elevated resistance not only to norfloxacin but also to acriflavine, 4′,6‐diamidino‐2‐phenylindole, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, Hoechst 33342, daunomycin, berberine, and sodium deoxycholate in Escherichia coli KAM32, a drug‐hypersensitive strain. We observed an Na + ‐dependent efflux of ethidium and an ethidium‐induced efflux of Na + in E. coli KAM32 cells harboring the plasmid carrying the hmrM gene. These results indicate that HmrM is an Na + /drug antiporter‐type multidrug efflux pump. A difference in substrate preference was observed between HmrM, NorM, and YdhE.