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Depolymerization of Actin Filament by Cytochalasin E Induces Interleukin‐8 Production and Up‐Regulates CD54 in the HeLa Epithelial Cell Line
Author(s) -
Ikewaki Nobunao,
Yamada Akira,
Inoko Hidetoshi
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb03435.x
Subject(s) - cytochalasin d , cytochalasin , biology , cytochalasin b , microbiology and biotechnology , hela , actin , cell culture , cytoskeleton , cell , biochemistry , genetics
We previously reported that the depolymerization of actin filament by cytochalasin E enhances low affinity Fcε receptor II (CD23) expression on the human monocyte‐like cell line, U937 (J. Clin. Immunol. 20: 235, 2000). In this study, we found that cytochalasin E strongly induces interleukin‐8 through an epithelial cell line, HeLa, in dose‐ and time‐dependent manners as assessed by enzyme‐linked immunoassay and reverse transcription‐polymerase chain reaction techniques. In addition, interleukin‐8 production in the HeLa cells cultured with cytochalasin E was blocked in the presence of protein kinase C inhibitors, Go6976 and H‐7. On the other hand, it was found that CD54 (intercellular adhesion molecule‐1; ICAM‐1) expression on the HeLa cells and the secretion of soluble CD54 were significantly up‐regulated after culturing with cytochalasin E, and that these up‐regulations of CD54 were also suppressed by Go6976. Taken together, these findings indicate that cytochalasin E activates protein kinase C under the depolymerization of actin filament, leading to the induction of interleukin‐8 production and the up‐regulation of CD54 in HeLa cells.