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T‐Cell Receptor Variable γ Chain Gene Expression in the Interaction between Rat γδ‐Type T Cells and Heat‐Shock Protein 70‐Like Molecule
Author(s) -
Ichinohe Takashi,
Ichimiya Shingo,
Kishi Akihiko,
Tamura Yasuaki,
Kondo Nobuhiko,
Ueda Gosei,
Torigoe Toshihiko,
Yamaguchi Akira,
Hiratsuka Hiroyoshi,
Hirai Itaru,
Kohama Genniku,
Sato Noriyuki
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb03406.x
Subject(s) - t cell receptor , biology , t cell , gene , heat shock protein , receptor , microbiology and biotechnology , amino acid , cell , nucleotide , biochemistry , genetics , immune system
We previously reported that rat T‐cell receptor (TCR) Vδ6 of T‐cell hybridomas was preferentially involved in recognition of the cell surface‐expressed 70 kDa rat heat‐shock cognate (hsc70, a constitutively expressed member of the hsp 70 family) protein‐like molecule (#067 molecule). In the present study, we analyzed usage of the TCR Vγ family of #067‐restricted T‐cell hybridomas. Our data indicated that most of these hybridomas expressed transcripts of TCR Vγ1 and/or Vγ2. However, some of the Vγ2 transcripts were out‐of‐frame, suggesting that the TCR Vγ1 family may be important for the recognition of #067‐defined molecules. TCR Vγ1 transcripts were detected in not only #067‐restricted T‐cell hybridomas, but #067‐non restricted ones as well. However, V‐J nucleotide sequences of #067‐restricted and #067‐non restricted T‐cell hybridomas suggested that #067‐restricted T‐cell hybridomas showed limited insertion of nucleotide stretch as compared with #067‐non restricted ones. In terms of amino acids, only one amino acid was added in #067‐restricted T‐cell hybridomas, whereas two or three amino acids were added in #067‐non restricted ones. These data suggest that the heterodimer of the TCR relatively short stretch form of Vγ1 molecule and TCR Vδ6 may participate in recognition of the #067 molecule.