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A Novel Murine Model of Oral Candidiasis with Local Symptoms Characteristic of Oral Thrush
Author(s) -
Takakura Natsuko,
Sato Yuichi,
Ishibashi Hiroko,
Oshima Haruyuki,
Uchida Katsuhisa,
Yamaguchi Hideyo,
Abe Shigeru
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb03403.x
Subject(s) - candida albicans , tongue , oral cavity , corpus albicans , biology , microbiology and biotechnology , oral administration , population , nystatin , inoculation , antifungal , medicine , pharmacology , immunology , pathology , dentistry , environmental health
A conventional and easy method to establish a murine oral candidiasis model, which has not only a stable yeast population in the oral cavity but also symptoms characteristic of oral thrush, was developed by using a sedative agent. Mice were immunosuppressed with prednisolone and were given tetracycline hydrochloride. They were orally infected with 10 6 viable cells of Candida albicans by means of a cotton swab and enough chlorpromazine chloride had been injected to keep them in a sedative state about for 3 hr after inoculation. From day 3 to day 7 post inoculation, 10 5 –10 6 colony forming units of Candida were recovered from the oral cavity of each mouse and whitish, curd‐like patches were observed on most parts of tongue. Microscopically, germ tubes had appeared on the tongue surface. This model would be a useful experimental oral candidiasis for investigating the pathogenesis of C. albicans oral infection and the efficacy of various antifungal agents microbiologically and symptomatically.