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Cell‐Mediated Fas‐Based Lysis of Dendritic Cells Which Are Apparently Resistant to Anti‐Fas Antibody
Author(s) -
Yokota Aya,
Oikawa Atsuhiko,
Matsuda Chieko,
Shinohara Nobukata,
Eshima Koji
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb03397.x
Subject(s) - lysis , biology , apoptosis , antibody , antigen , microbiology and biotechnology , programmed cell death , immunology , biochemistry
In this report, the controversy concerning the sensitivity of dendritic cells (DCs) to Fas‐dependent induction of apoptosis was examined using murine DCs. Although DCs could not be lysed when exposed to an anti‐Fas antibody, Jo2, the observed resistance turned out to reflect their lack of the expression of FcγR necessary for crosslinking the antibody, rather than their intrinsic resistance. Thus, at least a fraction of DCs was sensitive to Jo2 in the presence of FcγR‐expressing by‐standers. Consistently, a significant fraction of DCs was sensitive to Fas‐dependent lysis mediated by T cells including the antigen‐specific killing by CD4 + T cells. Both immature (class II MHC low ) and mature (class II MHC high ) DCs were sensitive to the Fas‐based induction of apoptosis.