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An Effective Sporicidal Reagent against Bacillus subtilis Spores
Author(s) -
Kida Nori,
Mochizuki Yasushi,
Taguchi Fumiaki
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb03396.x
Subject(s) - reagent , ethylenediaminetetraacetic acid , bacillus subtilis , ethanol , sodium hypochlorite , spore , chemistry , chromatography , nuclear chemistry , endospore , salt (chemistry) , hypochlorite , microbiology and biotechnology , bacteria , biology , biochemistry , organic chemistry , chelation , genetics
We developed a reagent which showed significant sporicidal activity against Bacillus subtilis spores. This reagent was composed of ethylenediaminetetraacetic acid, disodium salt (EDTA‐2Na), ferric chloride hexahydrate (FeCl 3 · 6H 2 O) and ethanol (tentatively designated as the ethanol reagent). The ethanol reagent showed pH‐ and temperature‐dependent sporicidal activity. At pH 0.3, its activity was almost the same as that of 0.05% sodium hypochlorite at 20 C and was higher at 37 C than at 20 C. The activity of the ethanol reagent was similar both with and without 10% serum. The ethanol reagent might be applicable for disinfecting Bacillus spores.