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Role of Microfilaments and Microtubules in the Invasion of INT‐407 Cells by Campylobacter jejuni
Author(s) -
Biswas Debabrata,
Itoh Kikuji,
Sasakawa Chihiro
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb03372.x
Subject(s) - microfilament , internalization , nocodazole , microtubule , biology , cytochalasin d , cytochalasin b , cytochalasin , cytoskeleton , depolymerization , microbiology and biotechnology , campylobacter jejuni , cell , biochemistry , chemistry , genetics , bacteria , organic chemistry
The internalization mechanisms triggered by Campylobacter jejuni were studied by invasion assays conducted with different inhibitors that act on the cytoskeleton structure of eukaryotic cells. The depolymerization of microfilaments by cytochalasin‐D and that of microtubules by colchicines and nocodazole inhibited the uptake of C. jejuni into INT‐407 cells in a dose‐dependent manner. The inhibitory effect of microfilament depolymerization on C. jejuni internalization was more pronounced than that of microtubule depolymerization. By immunofluorescence microscopic observations, it was demonstrated that both microfilaments and microtubules were localized in INT‐407 cells after C. jejuni infection. These data suggest that the internalization mechanism triggered by C. jejuni is associated with the combined effect of microfilaments and microtubules of host cells.