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Screening Method for Salmonella enterica Serovar Typhi and Serovar Paratyphi A with Reduced Susceptibility to Fluoroquinolones by PCR‐Restriction Fragment Length Polymorphism
Author(s) -
Hirose Kenji,
Tamura Kazumichi,
Watanabe Haruo
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb02800.x
Subject(s) - serotype , biology , salmonella enterica , restriction fragment length polymorphism , microbiology and biotechnology , ciprofloxacin , salmonella typhi , virology , salmonella , polymerase chain reaction , gene , genetics , bacteria , antibiotics , escherichia coli
Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 μg/ml) have a mutation at codon either Ser‐83 or Asp‐87 of gyrA gene. A screening method by PCR‐restriction fragment length polymorphism (PCR‐RFLP) was designed to screen the mutations at codon Ser‐83 and Asp‐87 of the gyrA gene of S. enterica serovar Typhi and serovar Paratyphi A clinical isolates. This method successfully screened the gyrA mutations of S. enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones.