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Relationship between Canine Mucosal and Serum Immunoglobulin A (IgA) Concentrations: Serum IgA Does Not Assess Duodenal Secretory IgA
Author(s) -
Rinkinen Minna,
Teppo Annamaija,
Harmoinen Jaana,
Westermarck Elias
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb02799.x
Subject(s) - immunoglobulin a , saliva , secretory iga , secretory component , immunoassay , antibody , biology , immunoglobulin g , medicine , j chain , immunology
A double‐sandwich enzyme immunoassay method was developed for determination of serum immunoglobulin A (S‐IgA) and mucosal secretory immunoglobulin A (sIgA) in duodenal brush samples obtained via endoscopy and the relationship between enteric mucosal sIgA, salivary sIgA and S‐IgA in dogs was examined. Twenty healthy dogs underwent routine endoscopy. A brush sample from the duodenal mucosa was obtained and washed in PBS, with a serum sample being taken concurrently. A saliva sample was collected from twelve of these dogs. S‐IgA and sIgA with total protein concentrations in the duodenal washings and saliva samples were determined. A significant negative correlation ( r = −0.64, P = 0.0059) was found between duodenal sIgA/protein ratios and S‐IgA concentrations. Saliva sIgA/protein ratios did not correlate with sIgA/protein ratios of duodenal samples. The method described here allows for direct assessment of duodenal IgA; therefore indirect measures based on serum IgA or salivary IgA can be avoided. In addition, these indirect measures appear to be poor indicators of duodenal sIgA competence in dogs.