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Adhesion and Colonization of Enterohemorrhagic Escherichia coli O157:H7 in Cecum of Mice
Author(s) -
Nagano Keiji,
Taguchi Kazuki,
Hara Takumi,
Yokoyama Shinichiro,
Kawada Kenji,
Mori Hiroshi
Publication year - 2003
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2003.tb02795.x
Subject(s) - microbiology and biotechnology , cecum , biology , escherichia coli , feces , diarrhea , bacteria , colonization , pathogen , secretion , enterobacteriaceae , mutant , epithelium , virology , gene , pathology , medicine , ecology , biochemistry , genetics
Infectious diseases due to enterohemorrhagic Escherichia coli (EHEC) are characterized by diarrhea, hemorrhagic colitis and hemolytic uremic syndrome. The adherence of EHEC on intestinal epithelial cells is a first step for developing these diseases. In the present study, we examined whether EHEC O157: H7 adhere to intestinal epithelial cells of mice and cause F‐actin accumulation in the epithelial cells following the intragastric inoculation of the pathogen. Fecal shedding of the EHEC O157:H7 strain was observed in ICR mice up to 3 weeks. Fecal shedding periods of the type III secretion system‐related gene ( espA and sepL ) deletion mutants were clearly shorter than that of the wild‐type EHEC O157:H7 strain. The EHEC O157:H7 colonies were found on the epithelial surfaces of the ceca in association with F‐actin accumulation beneath the attached bacteria.