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Distribution of Aeromonas hydrophila Serogroups in Different Clinical Samples and the Development of Polyclonal Antibodies for Rapid Identification of the Genus Aeromonas by Direct Agglutination
Author(s) -
Korbsrisate Sunee,
Dumnin Suwat,
Chawengkirttikul Rungrawan,
Gherunpong Vanchit,
Eampokalap Boonchuay,
Gongviseisoog Charles,
Janyapoon Kritsana,
Lertpocasombat Kancharee,
Shimada Toshio
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02775.x
Subject(s) - aeromonas , aeromonas hydrophila , biology , agglutination (biology) , microbiology and biotechnology , direct agglutination test , antiserum , polyclonal antibodies , serotype , bacteria , antibody , serology , immunology , genetics
We characterized a collection of 256 Aeromonas hydrophila strains isolated from blood, discharge and stool for their serogroup designation. Of these, 2.3% were untypable and 15.2% were rough strains. Among the typable strains, about 50% comprised serogroups O:11, O:16, O:18, O:34 and O:83. To develop rapid differentiation of Aeromonas from other oxidase‐positive bacteria, antisera against Aeromonas were produced to establish a direct, genus‐specific, agglutination test. It was found that among 105 isolates of Aeromonas , 102 showed positive results with the agglutination test. The calculated sensitivity and specificity were 97.1% and 90.7%, respectively.