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Interferon Regulatory Factor 1 in Mycobacterial Infection
Author(s) -
Yamada Hiroyuki,
Mizuno Satoru,
Sugawara Isamu
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02760.x
Subject(s) - biology , knockout mouse , spleen , tuberculosis , pathogenesis , mycobacterium tuberculosis , immunology , interferon gamma , tumor necrosis factor alpha , interleukin 10 , interferon regulatory factors , messenger rna , cytokine , pathology , gene , immune system , medicine , innate immune system , biochemistry
In order to understand the role of IRF‐1 in the development of murine tuberculosis in vivo , IRF‐1 knockout mice were infected with Mycobacterium tuberculosis by placing them in the exposure chamber of an airborne infection apparatus. These knockout mice developed multifocal necrotic lesions in the lung, liver and spleen tissues and died of disseminated tuberculosis within 43 days of infection. Compared with the levels in wild‐type mice, the pulmonary inducible NO synthase (iNOS) mRNA expression level was significantly lower, but IL‐18 and IL‐6 mRNA levels were higher. There was no statistically significant difference in the expression of IFN‐γ and TNF‐α mRNA between the IRF‐1 knockout and wild‐type mice. IRF‐1 is indirectly responsible for iNOS mRNA expression and plays an important role in the pathogenesis of murine tuberculosis.