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Characterization of Borrelia afzelii Isolated from Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, by PCR‐RFLP Analyses of ospC Gene and rrf (5S)‐ rrl (23S) Intergenic Spacer
Author(s) -
Lee Seunghyun,
Jung Kwangdon,
Lee Junghee,
Kim Sangchoin,
Kim Jonghyun,
Jang Wonjong,
Park Kyunghee
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02751.x
Subject(s) - biology , restriction fragment length polymorphism , apodemus agrarius , genetics , microbiology and biotechnology , sensu , virology , gene , zoology , polymerase chain reaction , ecology , rodent , genus
Nine Borrelia afzelii strains, which had been isolated from two vectors, Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, were characterized by PCR‐RFLP analyses of ospC genes and rrf (5S)‐ rrl (23S) intergenic spacer. Dra I restriction patterns of Chungju strains were identical to those of B. afzelii VS461. But Mse I restriction patterns of rrf (5 S)‐rrl (23S) intergenic spacer genes of KK2, KM4, KK5 differed from those of previously reported B. burgdorferi sensu lato strains. Nine Chungju strains were classified with four distinct ospC RFLP patterns, which differed from the eight ospC RFLP patterns (A‐1 to A‐8) of previously reported B. afzelii . Moreover, five additional restriction patterns were deduced from published ospC sequences of reference strains. These results suggest that Chungju strains are very heterogeneous.