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Detection of Specific Helicobacter pylori DNA and Antigens in Stool Samples in Dyspeptic Patients and Healthy Subjects
Author(s) -
Wiśniewska Monika,
Nilsson Hans Olof,
BąlkRomaniszyn Leokadia,
Rechciński Tomasz,
Bielański Władysław,
PłanetaMałecka Izabela,
Płonka Małgorzata,
Konturek Stanisław,
Wadström Torkel,
Rudnicka Wiesława,
Chmiela Magdalena
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02749.x
Subject(s) - helicobacter pylori , urea breath test , serology , antigen , rapid urease test , biology , feces , spirillaceae , gastroenterology , helicobacter , polymerase chain reaction , medicine , immunology , microbiology and biotechnology , gastritis , helicobacter pylori infection , antibody , gene , biochemistry
In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi‐nested PCR ( ureA ‐PCR), respectively. The H. pylori status was estimated by invasive endoscopy‐based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of H. pylori ‐negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA ‐PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one‐step diagnosis of H. pylori infection.