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Development of an Immunofluorescence Method for the Detection of Antibodies to Ebola Virus Subtype Reston by the Use of Recombinant Nucleoprotein‐Expressing HeLa Cells
Author(s) -
Ikegami Tetsuro,
Saijo Masayuki,
Niikura Masahiro,
Miranda Mary E.G.,
Calaor Alan B.,
Hernandez Marvin,
Manalo Daria L.,
Kurane Ichiro,
Yoshikawa Yasuhiro,
Morikawa Shigeru
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02745.x
Subject(s) - nucleoprotein , virology , antibody , hela , biology , immunofluorescence , ebola virus , virus , recombinant dna , ebolavirus , microbiology and biotechnology , cell culture , immunology , gene , genetics , biochemistry
An indirect immunofluorescent assay (IFA) to detect Ebola virus subtype Reston (EBO‐R) antibodies was developed by the use of a HeLa cell line stably expressing EBO‐R nucleoprotein (NP). This IFA has a high specificity for the detection of EBO‐R IgG antibodies in both hyperimmune rabbit sera and monkey sera collected during an EBO‐R outbreak in the Philippines in 1996. Furthermore, this IFA showed a higher sensitivity for the detection of EBO‐R antibodies than did the IFA using HeLa cells expressing the NP of Ebola virus subtype Zaire. These results suggest that this new IFA is useful for seroepidemiological studies of EBO‐R infection among monkeys.