Premium
Effects of Recombinant Cholera Toxin B Subunit on IL‐1β Production by Macrophages In Vitro
Author(s) -
Maeyama Junichi,
Isaka Masanori,
Yasuda Yoko,
Matano Keiko,
Taniguchi Tooru,
Morokuma Kazunori,
Ohkuma Kunio,
Tochikubo Kunio,
Goto Norihisa
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02740.x
Subject(s) - biology , cholera toxin , microbiology and biotechnology , peptidoglycan , in vitro , adjuvant , intracellular , secretion , recombinant dna , stimulation , biochemistry , gene , immunology , neuroscience
Recombinant cholera toxin B subunit (rCTB) is a safe and potent mucosal adjuvant. As a clue to the mechanism of the adjuvant effect of rCTB, the profile of cytokines secreted in vitro by the mouse peritoneal macrophage (MΦ) treated with rCTB was examined. IL‐1β secretion, intracellular production, and expression of its mRNA of LPS‐stimulated MΦ was greatly enhanced by treatment with rCTB. IL‐1β production in response to other microbial stimulators, such as Pansorbin, Sansorbin, insoluble peptidoglycan, and Taxol, was also potentiated by rCTB. MΦ pretreated with rCTB before 24 hr could maintain the ability to produce a high level of IL‐1β, suggesting that this ability may be involved in the adjuvant activity of rCTB on MΦ stimulation. The possibility of close association between rCTB and signal transduction of a Toll‐like receptor family in MΦ is discussed.