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Menadione‐Catalyzed Luminol Chemiluminescent Assay for Viability of Mycobacterium bovis
Author(s) -
Yamashoji Shiro
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02735.x
Subject(s) - menadione , chemiluminescence , luminol , biology , mycobacterium bovis , microbiology and biotechnology , virology , mycobacterium tuberculosis , biochemistry , chromatography , chemistry , tuberculosis , medicine , enzyme , pathology
Stable luminol chemiluminescence was observed 10 min after the addition of menadione to a suspension of Mycobacterium bovis homogenized in Middlebrook 7H9 broth base including OADC enrichment. The chemiluminescence intensity was proportional to the absorbance of the bacterial suspension at 600 nm in a range of 0.005 to 0.15. Luminol chemiluminescence disappeared after 10 min incubation of M. bovis at over 60% of ethanol or 4 days of cultivation of M. bovis in the presence of 40 μg/ml of streptomycin. The bacterium showing the disappearance of chemiluminescence could not grow after being washed, suggesting that the inhibition concentration of the antimicrobials can be estimated on the basis of the disappearance of chemiluminescence. Menadione‐catalyzed luminol chemiluminescent assay was rapid and sensitive in comparison to turbidimetry, tetrazolium (WST‐8) reduction assay, and the assay using the Mycobacteria growth indicator tube (MGIT).