Premium
Isolation and Genetic Characterization of a Novel Filamentous Bacteriophage, a Deleted Form of Phage f237, from a Pandemic Vibrio parahaemolyticus O4:K68 Strain
Author(s) -
Chang Bin,
Miyamoto Hiroshi,
Taniguchi Hatsumi,
Yoshida Shinichi
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02734.x
Subject(s) - biology , microbiology and biotechnology , bacteriophage , vibrio parahaemolyticus , virology , lysogenic cycle , vibrio cholerae , open reading frame , strain (injury) , serotype , plasmid , gene , genetics , bacteria , escherichia coli , peptide sequence , anatomy
We isolated a filamentous bacteriophage, VfO4K68, from the pandemic Vibrio parahaemolyticus strain belonging to O4:K68 serovar. The VfO4K68 DNA lacked a 1,893‐bp fragment present in that of the distinctive region of f237, a filamentous phage isolated from a pandemic O3:K6 strain (Nasu, H. et al., J. Clin. Microbiol., 38, 2156–2161, 2000). The deletion resulted in the formation of a novel open reading frame (ORF) that possesses homology to the ORF 27 of ETA phage and staphylococcal enterotoxin E (SEE) of Staphylococcus aureus . VfO4K68 was able to infect the recipient O3:K6 serovar strains. These results suggest that VfO4K68 might act as a genetic transmitter and play some roles in the pandemic V. parahaemolyticus infection.