Premium
An Application of Microplate Hybridization Assay for the Confirmation and Probe Typing of “Norwalk‐Like Viruses”
Author(s) -
Fukuda Shinji,
Takao Shinichi,
Shimazu Yukie,
Miyazaki Kazuo
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02725.x
Subject(s) - norwalk virus , biology , typing , virology , microbiology and biotechnology , genetics , virus , norovirus
“Norwalk‐like virus” (NLV) genomes are generally detected by using reverse transcription‐PCR and confirmed by blot hybridization and nucleotide sequencing because of their fastidious nature. In the present study, the confirmation and typing of NLV genomes were carried out using a streptavidin‐biotin binding technique and microplate hybridization assay with digoxigenin labeled probes. Eight probe typing sets (G1A, G1B, G2A, G2B, G2C, G2D, G2E, and G2F) formatted from 6 newly designed probes and 8 probes reported elsewhere were used for hybridization. The correlation between probe typing and nucleotide sequencing was found and our 8 probe sets were useful for the typing of NLVs.