Premium
Additional Inhibitory Effect of Tea Extract on the Growth of Influenza A and B Viruses in MDCK Cells
Author(s) -
Imanishi Nobuko,
Tuji Yumiko,
Katada Yuko,
Maruhashi Miyuki,
Konosu Satoko,
Mantani Naoki,
Terasawa Katutoshi,
Ochiai Hiroshi
Publication year - 2002
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2002.tb02724.x
Subject(s) - biology , green tea extract , endosome , virus , influenza a virus , inhibitory postsynaptic potential , catechin , intracellular , growth inhibition , virology , green tea , polyphenol , biochemistry , cell growth , food science , antioxidant , neuroscience
It has been previously reported that green‐tea extract (GTE) inhibits the growth of influenza virus by preventing its adsorption. In this study, we further investigated whether GTE exerts an additional inhibitory effect on the acidification of intracellular compartments such as endosomes and lysosomes (referred to as ELS) and thereby inhibits the growth of influenza A and B viruses in Madin‐Darby canine kidney cells. The vital fluorescence microscopic study showed that GTE inhibited acidification of ELS in a concentration‐dependent manner. Moreover, the growth of influenza A and B viruses was equally inhibited when the cells were treated with GTE within as early as 5 to 15 min after infection, depending on the virus strains. The fact that (—)epigallocatechin (EGC), one of major catechin molecules in GTE, exerts the inhibitory effects on the acidification of ELS and virus growth in a manner similar to that of GTE strongly suggests that EGC is one of the active components in the extract.