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Identification of a H + /Glucose and Galactose Symporter Gene glt from Xanthomonas oryzae pv. oryzae
Author(s) -
Tsuge Seiji,
Furutani Ayako,
Kubo Yasuyuki,
Horino Osamu
Publication year - 2001
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2001.tb02656.x
Subject(s) - symporter , xanthomonas oryzae , biology , xanthomonas oryzae pv. oryzae , escherichia coli , biochemistry , galactose , cotransporter , glucose transporter , gene , transporter , microbiology and biotechnology , chemistry , sodium , organic chemistry , insulin , endocrinology
We identified a glucose and galactose transporter gene from the plant‐pathogenic bacterium Xanthomonas oryzae pv. oryzae . Sequence analysis indicated that the gene, named glt , encoded a polypeptide of 592 amino acid residues and the product was significantly homologous with members of the Na + /glucose cotransporter (SGLT) family from mammalian and bacterial origin, especially with vSGLT from Vibrio parahaemolyticus (50% identity). GLT functioned as a glucose and galactose transporter in an Escherichia coli mutant deficient in glucose and galactose transport activity. A protonophore inhibited the transport activity, suggesting that GLT is a H + ‐coupled glucose/galactose symporter.