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Fate of Legionella pneumophila in Macrophages of C57BL/6 Chronic Granulomatous Disease Mice
Author(s) -
Saito Mitsumasa,
Kajiwara Hideko,
Miyamoto Hiroshi,
Yoshida Shinichi
Publication year - 2001
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2001.tb02655.x
Subject(s) - chronic granulomatous disease , legionella pneumophila , phagocytosis , microbiology and biotechnology , biology , superoxide , effector , macrophage , in vitro , intracellular , intracellular parasite , immunology , bacteria , biochemistry , enzyme , genetics
We compared the intracellular survival and growth of Legionella pneumophila Philadelphia‐1 in peritoneal macrophages obtained from A/J, C57BL/6, and X‐linked chronic granulomatous disease (CGD) mice produced from C57BL/6 strain. The initial killing was observed in A/J and C57BL/6 macrophages at 2,4 and 6 hr after in vitro phagocytosis, but not in the CGD macrophages. Thereafter, there was a 10‐fold increase of CFU in A/J macrophages. The bacteria, however, did not proliferate in C57BL/6 and CGD macrophages at 24 or 48 hr after in vitro phagocytosis. These results suggest that effector molecules for the initial killing are a superoxide anion and its metabolites, and Lgn1 gene product inhibits the intracellular growth of L. pneumophila independently of NADPH oxidase.