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Structural Characterization of the O‐Antigenic Polysaccharide Chain of Porphyromonas circumdentaria NCTC 12469
Author(s) -
Matsuo Kouki,
Isogai Emiko,
Araki Yoshio
Publication year - 2001
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2001.tb02622.x
Subject(s) - polysaccharide , antiserum , galactose , acid hydrolysis , biochemistry , porphyromonas gingivalis , antigen , biology , hydrolysis , stereochemistry , chemistry , bacteria , genetics
Structural studies were carried out on an O‐antigenic polysaccharide moiety derived from Porphyromonas circumdentaria NCTC 12469, a reference strain of Porphyromonas species. The polysaccharide chain was composed of d ‐glucose, d ‐galactose, N ‐acetyl‐ d ‐glucosamine, and N ‐acetyl‐ d ‐galactosamine in a molar ratio of 1:2:1:1. On the basis of results from 1 H‐ and 13 C‐NMR spectroscopic analyses including COSY, TOCSY, and HMQC experiments together with results of Smith degradation, methylation analysis, and partial acid hydrolysis, it is concluded that the polysaccharide chain has a pentasaccharide repeating unit of →6)‐β‐ d ‐Glc p ‐(1→6)‐β‐ d ‐Gal p ‐(1→6)‐β‐ d ‐Gal p ‐(1→3)‐β‐ d ‐Glc p NAc‐(1→3)‐β‐ d ‐Gal p NAc‐(1→. The immunoreaction between P. circumdentaria LPS and the corresponding antiserum was strongly inhibited by the pentasaccharide fragment (Glc‐Gal‐Gal‐GlcNAc‐GalNAc) isolated from partial acid hydrolysis of the above polysaccharide, suggestive of O‐antigen specific antibodies in the used antiserum.