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Effect of Sealing and Tween 80 on the Antifungal Susceptibility Testing of Essential Oils
Author(s) -
Inouye Shigeharu,
Tsuruoka Tsutomu,
Uchida Katsuhisa,
Yamaguchi Hideyo
Publication year - 2001
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2001.tb02608.x
Subject(s) - aspergillus fumigatus , agar dilution , agar , candida albicans , dilution , microbiology and biotechnology , biology , agar plate , candida parapsilosis , agar dilution method , food science , aspergillus , chromatography , minimum inhibitory concentration , bacteria , antibiotics , chemistry , genetics , thermodynamics , physics
Concentrations of essential oils showing high volatility decreased substantially in broth and agar media when incubated under open conditions. The decrease in the half life was from 0.7 to 38 hr in broth medium at 27 C. When evaporation was prevented by sealing, MIC values against Aspergillus fumigatus, Candida albicans and Trichophyton mentagrophytes by broth or agar dilution assay were lowered two to eight‐fold, as compared with those obtained under open conditions. Addition of Tween 80 caused a rise of the MICs against A. fumigatus by two to four‐fold in broth dilution assay, but little affected the MICs in agar dilution assay.