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Detection of Virulence Associated Genes in Clinical Strains of Vibrio mimicus
Author(s) -
Bi Kewei,
Miyoshi Shinichi,
Tomochika Kenichi,
Shinoda Sumio
Publication year - 2001
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2001.tb01292.x
Subject(s) - virulence , biology , gene , microbiology and biotechnology , polymerase chain reaction , genetics
A total of 42 clinical strains of Vibrio mimicus were examined for the presence of virulence associated genes toxR, toxS, toxT, tcpP, ctx and tcpA by PCR assay. Almost all strains were shown to have the toxR gene, while the toxS gene was found in 27 strains. On the other hand, five strains possessed both toxT and tcpP genes, but others had neither. Only two strains were positive for amplification of the ctx gene, whereas no PCR product with tcpA primers was detected. The results indicate the incomplete copies of virulence cascade in V. mimicus strains. The pathogenesis and epidemic potential of this species is also discussed.