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Use of Antiserum‐Coated Latex Particles for Serotyping Streptococcus pneumoniae
Author(s) -
Arai Satoru,
Konda Toshifumi,
Wada Akihito,
Matsunaga Yasuko,
Okabe Nobuhiko,
Watanabe Haruo,
Inouye Sakae
Publication year - 2001
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2001.tb01284.x
Subject(s) - antiserum , serotype , streptococcus pneumoniae , latex fixation test , microbiology and biotechnology , agglutination (biology) , direct agglutination test , biology , virology , antibody , immunology , serology , antibiotics
The Quellung reaction provides a standard means for serotyping Streptococcus pneumoniae , but it requires microscopic examination with skillful technique. We have developed an improved agglutination method with anti‐rabbit IgG‐coated latex particles, which are sensitized with pooled antisera for serotyping/serogrouping S. pneumoniae . Our method is as specific and sensitive as the Quellung test, and much easier to perform.