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Genomic Map of Clostridium perfringens Strain 13
Author(s) -
Shimizu Tohru,
Ohshima Shinobu,
Ohtani Kaori,
Shimizu Takeshi,
Hayashi Hideo
Publication year - 2001
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2001.tb01278.x
Subject(s) - clostridium perfringens , biology , plasmid , strain (injury) , genetics , circular bacterial chromosome , virulence , chromosome , gene , genome , gene mapping , microbiology and biotechnology , bacteria , anatomy
A physical and genetic map of Clostridium perfringens strain 13 was constructed. C. perfringens strain 13 was found to have a 3.1‐Mb chromosome and a large 50‐kb plasmid, indicating that strain 13 has a relatively small genome among C. perfringens strains. A total of 313 genetic markers were mapped on the chromosome of strain 13. Compared with the physical and genetic map of C. perfringens CPN50, strain 13 had a quite similar genome organization, but with a large deletion (~400 kb) in a particular segment of the chromosome. Among several toxin genes, a beta2 toxin gene that is a novel virulence gene in C. perfringens was found to be located on the 50‐kb plasmid.