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Molecular Analysis of the folC Gene of Pseudomonas aeruginosa
Author(s) -
Murata Takahiro,
Bognar Andrew L.,
Hayashi Tetsuya,
Ohnishi Makoto,
Nakayama Keisuke,
Terawaki Yoshiro
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02578.x
Subject(s) - gene , biology , pseudomonas aeruginosa , genetics , escherichia coli , nucleic acid sequence , coding region , gene cluster , microbiology and biotechnology , bacteria
We have cloned the Pseudomonas aeruginosa folC gene coding for folylpolyglutamate synthetase‐dihydrofolate synthetase, which was located between the trpF and purF loci, and determined the nucleotide sequence of the folC gene and its flanking region. The deduced amino acid sequence of P. aeruginosa FolC was highly homologous to that of Escherichia coli FolC. The cloned gene complemented E. coli folC mutations and was found to encode both folylpolyglutamate synthetase and dihydrofolate synthetase activities. The gene organization around the folC gene in P. aeruginosa was completely conserved with that in E. coli ; the accD gene was located upstream of the folC gene, and dedD, cvpA and purF genes followed the folC gene in this order. The gene arrangement and the result of the promoter activity assay suggested that the P. aeruginosa accD and folC genes were co‐transcribed.