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Erythromycin Resistance Genes in Streptococcus pyogenes Isolates in Kanagawa, Japan
Author(s) -
Murase Toshiyuki,
Suzuki Rieko,
Watanabe Yuko,
Yamai Shiro
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02575.x
Subject(s) - streptococcus pyogenes , biology , erythromycin , microbiology and biotechnology , agar dilution , serotype , clone (java method) , pulsed field gel electrophoresis , streptococcaceae , strain (injury) , agar dilution method , antibiotics , gene , minimum inhibitory concentration , genotype , bacteria , genetics , staphylococcus aureus , anatomy
The susceptibility of 224 Streptococcus pyogenes isolates obtained from children in Japan from 1981 to 1997 to treatment with erythromycin was determined by the agar dilution method. A total of 17 isolates belonging to serotype M12T12 were resistant (MICs > 1 μg/ml). Fourteen of the 17 resistant strains obtained from 1982 to 1985 harbored ermB and showed an identical pulsed‐field gel electrophoresis pattern, indicating the spread of a single clone. Two ermTR ‐containing isolates were obtained in 1983. mefA gene was found in a strain obtained in 1994 in the present study, although this gene is predominantly associated with recent erythromycin resistance among S. pyogenes strains in many countries.