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Localization of Major, High Molecular Weight Proteins in Bacteroides forsythus
Author(s) -
Higuchi Naoya,
Murakami Yukitaka,
Moriguchi Keiichi,
Ohno Norikazu,
Nakamura Hiroshi,
Yoshimura Fuminobu
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02563.x
Subject(s) - immunogold labelling , immunoelectron microscopy , biology , western blot , cytoplasm , cell fractionation , bacteroides , bacteroides thetaiotaomicron , molecular mass , bacteroidaceae , antibody , microbiology and biotechnology , biochemistry , bacteria , immunology , gene , genetics , membrane , enzyme
Bacteroides forsythus produces species‐specific major proteins with high molecular weights of 270 and 230‐kDa (270K and 230K). A specific antibody raised against 270K was used for Western blot analysis and immunoelectron microscopy. Western blot analysis showed that the 270K and 230K proteins were immunologically similar. Immunogold labeling of ultrathin‐sectioned bacterial cells and biochemical fractionation revealed that these proteins were localized at the outermost cell surface, not in the cytoplasm. These results suggest that major proteins ubiquitous to this species may form the S‐layer.