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Adherence Protects the Binding Sites of Helicobacter pylori Urease from Acid‐Induced Damage
Author(s) -
Icatlo Faustino C.,
Yokoyama Hideaki,
Kuroki Masahiko,
Kobayashi Chizu,
Goshima Hideo,
Ikemori Yutaka,
Kodama Yoshikatsu
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02562.x
Subject(s) - urease , helicobacter pylori , mucin , enzyme , mucus , microbiology and biotechnology , spirillaceae , acetohydroxamic acid , biology , colonization , bacteria , biochemistry , gastritis , ecology , genetics
Colonization by Helicobacter pylori partly depends on acid‐dependent adherence by urease to gastric mucin. To further verify the relevance of urease adherence to colonization, the influence of acidity on the binding sites of H. pylori urease was investigated. When enzyme‐based in vitro ligand capture assays were used, the effect of acidity on the binding site of H. pylori urease was determined against a backdrop medium consisting of acidic buffers simulating the luminal side of gastric mucus. A high degree of stability was exhibited by adherent urease, suggesting a pivotal role by the denatured enzyme in the persistence of the bacterium within the acidified compartment of gastric mucus.