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Monoclonal Antibody against Mycoplasma fermentans ‐Specific Aminoglycoglycerolipid
Author(s) -
Matsuda Kazuhiro,
Li Jinliang,
Ichinose Shizuko,
Harasawa Ryo,
Saito Masaki,
Yamamoto Naoki
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02551.x
Subject(s) - immunoelectron microscopy , monoclonal antibody , biology , antigen , immunofluorescence , microbiology and biotechnology , mycoplasmataceae , phosphocholine , mycoplasma , immunostaining , antibody , immunogold labelling , staining , mollicutes , immunohistochemistry , biochemistry , immunology , phosphatidylcholine , phospholipid , genetics , membrane
Previously, we reported that Mycoplasma fermentans has specific antigens (phosphocholine‐containing glycoglycerolipids: GGPL‐I and GGPL‐III) and discussed the possibility of their pathogenic role. In this paper, we report the characterization of a monoclonal antibody (MF‐III‐1) specific to GGPL‐III (phosphocholine‐containing aminoglycoglycerolipid) using methods of electron microscopy, immunofluorescence cell surface staining, laser scanning microscopy, immunoelectron microscopy, and thin‐layer chromatography immunostaining. The MF‐III‐1 antibody specifically recognized M. fermentans attached to the surface of HTLV‐I‐infected human helper T‐cells, and it did not cross‐react with other lipids nor with human T‐cell antigens. Since MF‐III‐1 distinguishes GGPL‐III from GGPL‐I, the binding site may include a serinol (2‐amino‐1,3‐propanediol) residue of GGPL‐III. MF‐III‐1 is useful for the in vitro study of M. fermentans , and may also be useful as a tool for the study of the involvement of M. fermentans in human diseases.