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Detection of Microsporum canis in the Skin Scrapings and Hairs of Dogs with Dermatophytosis Based on Sequences of the Chitin Synthase 1 Gene
Author(s) -
Kano Rui,
Nakamura Yuka,
Watanabe Shinichi,
Hasegawa Atsuhiko
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02540.x
Subject(s) - microsporum canis , biology , chitin synthase , canis , gene , microbiology and biotechnology , chitin , genetics , biochemistry , ecology , antifungal , chitosan
In the present study, to confirm Microsporum canis infection rapidly, we detected the chitin synthase gene 1 ( CHS1 ) gene of M. canis in the hair and skin samples of four dogs with dermatophytosis. Amplification of the DNAs in the four samples with CHS1 primers yielded fragments of about 620‐bp. Nucleotide sequence analysis of the CHS1 gene fragments from samples and a reference strain of M. canis gene showed more than 99% similarity. The method presented in this study can rapidly detect the DNA of M. canis in skin scrapings, and we anticipate that it will be a useful microbiological tool for the diagnosis of M. canis infections in animals and humans.