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Genetic Analysis of the ycgJ‐metB‐cysK‐ygaG Operon Negatively Regulated by the VirR/VirS System in Clostridium perfringens
Author(s) -
Ohtani Kaori,
Takamura Hisao,
Yaguchi Harumi,
Hayashi Hideo,
Shimizu Tohru
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02528.x
Subject(s) - operon , biology , genetics , gene , regulon , clostridium perfringens , regulation of gene expression , mutant , bacteria
The 5′‐flanking region of the metB‐cysK‐ygaG operon, whose expression is negatively regulated by the VirR/VirS system in C. perfringens , was analyzed. The region contained the ycgJ, mscL , and colA genes encoding a hypothetical protein, a large conductance mechanosensitive channel protein, and kappa‐toxin (collagenase), respectively. Northern analysis revealed that the ycgJ gene was transcribed as a 4.9‐kb operon together with the metB‐cysK‐ygaG genes and that this operon was negatively regulated by the VirR/VirS system. It is indicated that the pfoA (theta‐toxin or perfringolysin O), colA , and ycgJ‐metB‐cysK‐ygaG genes that belong to the VirR/VirS regulon are situated very close together in a 26.5‐kb region of the chromosome, but do not form a pathogenic island.