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Effect of the Sialyl Lewis X (SLe) Moiety on Splenic Accumulation of SLe x ‐Carboxymethylpullulan Conjugate
Author(s) -
Horie Kazutoshi,
Sakagami Masahiro,
Kuramochi Kentaro,
Ito Teruomi,
Hamana Hiroshi
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02513.x
Subject(s) - conjugate , spleen , sialyl lewis x , lymphatic system , moiety , chemistry , microbiology and biotechnology , medicine , immunology , biology , endocrinology , stereochemistry , selectin , cell adhesion molecule , mathematics , mathematical analysis
Sialyl Lewis X (SLe x ), an E‐selectin ligand, was conjugated with carboxymethylpullulan (CMPul) and the disposition characteristics of this conjugate after intravenous administration were investigated using mice with ear edema. The concentration of 3 H‐labeled SLe x ‐CMPul in the spleen was significantly high. When CMPul was modified with a saccharide unable to bind to E‐selectin, this splenic accumulation was not observed. The uptake of radiolabeled SLe x ‐CMPul by the spleen was completely inhibited by a 100‐fold molar of cold SLe‐CMPul but not by a sialyl N ‐acetyllactosamine‐CMPul conjugate (SLN‐CMPul). Microautoradiography analyses revealed that SLe x ‐CMPul accumulated in the marginal zone of the spleen.