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Oxalate‐Degrading Enterococcus faecalis
Author(s) -
Hokama Sanehiro,
Honma Yasuko,
Toma Claudia,
Ogawa Yoshihide
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02489.x
Subject(s) - enterococcus faecalis , microbiology and biotechnology , bacteria , biology , oxalate , biochemistry , chemistry , staphylococcus aureus , organic chemistry , genetics
An oxalate‐degrading Enterococcus faecalis was isolated from human stools under anaerobic conditions. The bacteria required a poor nutritional environment and repeated subculturing to maintain their oxalate‐degrading ability. The E. faecalis produced 3 proteins (65, 48, and 40 kDa) that were not produced by non‐oxalate‐degrading E. faecalis as examined by SDS‐PAGE. Antibodies against oxalyl‐coenzyme A decarboxylase (65 kDa) and formyl‐coenzyme A transferase (48 kDa) obtained from Oxalobacter formigenes (an oxalate‐degrading anaerobic bacterium in the human intestine) reacted with 2 of the proteins (65 and 48 kDa) from the E. faecalis as examined by Western blottings. This is the first report on the isolation of oxalate‐degrading facultative anaerobic bacteria from humans.