Premium
An Exfoliative Toxin A‐Converting Phage Isolated from Staphylococcus aureus Strain ZM
Author(s) -
Yoshizawa Yukio,
Sakurada Junji,
Sakurai Susumu,
Machida Katsuhiko,
Kondo Isamu,
Masuda Shogo
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb02481.x
Subject(s) - lysogenic cycle , prophage , biology , microbiology and biotechnology , staphylococcal scalded skin syndrome , strain (injury) , staphylococcus aureus , toxin , gene , polymerase chain reaction , genetics , bacteriophage , bacteria , escherichia coli , anatomy
Exfoliative toxin A (ETA) causes staphylococcal scalded‐skin syndrome in children. The gene for ETA was believed to be coded by the chromosomal DNA. We isolated temperate phages from an ETA‐producing strain, ZM, using a restriction minus strain, 1039, as an indicator. One of the prophages, designated φ‐ZM‐1 mediated lysogenic conversion of ETA. The polymerase chain reaction assay of the eta gene revealed that phage φ‐ZM‐1 carries the structural gene for ETA.