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SDF‐1 Has Costimulatory Effects on Human T Cells
Author(s) -
Yonezawa Akihito,
Hori Toshiyuki,
Sakaida Hitoshi,
Uchiyama Takashi
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb01256.x
Subject(s) - biology , microbiology and biotechnology , mapk/erk pathway , stromal cell , t cell , mitogen activated protein kinase , progenitor cell , haematopoiesis , signal transduction , kinase , peripheral blood mononuclear cell , protein kinase a , immunology , stem cell , immune system , cancer research , biochemistry , in vitro
Stromal cell‐derived factor‐1 (SDF‐1) is an efficacious chemoattractant for lymphocytes, monocytes and hematopoietic progenitor cells. In the present study, we examined whether SDF‐1 has growth promoting activity on human peripheral T cells and analyzed the possible underlying signal transduction pathways. SDF‐1 augmented the proliferation of anti‐CD3‐ or PHA‐stimulated normal human PBMC in a dose‐dependent manner but not that of resting PBMC. It was noted that SDF‐1 alone could induce a significant proliferation of PHA‐preactivated T cells. Anti‐SDF‐1 sera could inhibit the augmentation of T‐cell proliferation in each experiment. Furthermore, Western blot analysis revealed that mitogen‐activated protein kinase (MAPK)/extracellular signal‐regulated kinase 2 (ERK2), but not c‐Jun N‐termimal kinase (JNK) was activated by SDF‐1. Considering that costimulatory signals have been reported to involve ERK2 activation, these results indicate that SDF‐1 has costimulatory effects on T cells that are possibly mediated by ERK2 activation and may play a role in not only migration but also the potentiation or maintenance of T cells.