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Efficient Induction of Peptide‐Specific Cytotoxic T Lymphocytes by LPS‐Activated Spleen Cells
Author(s) -
Kakugawa Kiyokazu,
Udaka Keiko,
Nakashima Kosuke,
Inaba Kayo,
Oka Yoshihiro,
Sugiyama Haruo,
Tamamura Hirokazu,
Yamagishi Hideo
Publication year - 2000
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.2000.tb01255.x
Subject(s) - cytotoxic t cell , biology , ctl* , antigen presenting cell , spleen , cd8 , antigen , microbiology and biotechnology , t cell , mhc class i , immunology , immune system , in vitro , biochemistry
Abstract Lipopolysaccharides of Gram‐negative bacteria are potent activators of B cells, dendritic cells and monocytes/macrophages. We have investigated the use of LPS‐activated spleen cells as antigen‐presenting cells to induce CD8 + cytotoxic T lymphocytes in vivo that are reactive to MHC class I binding peptides. Compared with resting spleen cells, CTL induction was more efficient and less variable for different peptides with LPS‐activated spleen cells. Cytotoxic responses were specific for the immunized peptides and contained high affinity CD8 + T cells. The removal of dendritic cells and monocytes/macrophages by Sephadex G10 column did not show profound effects on CTL induction, indicating that B‐cell blasts were largely responsible. This easily accessible method should facilitate the screening of MHC class I binding peptides to determine whether or not the host's T‐cell repertoire contains reactive T cells.