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Development of Restriction Fragment‐Length Polymorphism Method to Differentiate Five Subtypes of Feline Immunodeficiency Virus
Author(s) -
Kurosawa Kyoko,
Ikeda Yasuhiro,
Miyazawa Takayuki,
Izumiya Yoshihiro,
Nishimura Yorihiro,
Nakamura Kazuya,
Sato Eiji,
Mikami Takeshi,
Kai Chieko,
Takahashi Eiji
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02476.x
Subject(s) - biology , restriction enzyme , restriction fragment length polymorphism , feline immunodeficiency virus , virology , polymerase chain reaction , phylogenetic tree , group specific antigen , restriction fragment , genetics , virus , restriction site , microbiology and biotechnology , lentivirus , gene , viral disease
Feline immunodeficiency virus (FIV) isolates have been classified into five subtypes (A to E) based on the sequences of the env variable V3 to V5 region. In this study, we sequenced a partial gag region of 4 and 3 isolates belonging to subtypes C and E, respectively. Phylogenetic analysis revealed that the branching pattern based on the region was similar to that based on the env V3 to V5 region. Here, we propose a protocol to differentiate five subtypes by polymerase chain reaction amplifying 329 bp within the region followed by restriction fragment‐length polymorphism analysis using four restriction enzymes.