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Chitin Synthase 2 Gene Sequence of Malassezia Species
Author(s) -
Kano Rui,
Aizawa Tomohiro,
Nakamura Yuka,
Watanabe Shinichi,
Hasegawa Atsuhiko
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02475.x
Subject(s) - biology , chitin synthase , phylogenetic tree , malassezia , gene , nucleic acid sequence , genetics , genomic dna , polymerase chain reaction , chitin , microbiology and biotechnology , biochemistry , chitosan
Nucleotide sequences of the chitin synthase 2 ( CHS2 ) gene of seven species, Malassezia furfur, M. globosa, M. obtusa, M. pachydermatis, M. restricta, M. slooffiae and M. sympodialis , were analyzed for their phylogenetic relationship. About 620‐bp genomic DNA fragments of the CHS2 gene were amplified from these Malassezia species by polymerase chain reaction (PCR) and sequenced. The CHS2 nucleotide sequences of these Malassezia species showed more than 95% similarity between the species. A phylogenetic analysis of the nucleotide sequences of CHS2 gene fragments of seven Malassezia species revealed that the species were genetically distinct from each other.