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Human IgM Monoclonal Antibody to Ganglioside GM2 and Complement Suppress Virus Propagation in Ex Vivo Cultures of Lymphocytes from HIV‐1 Infected Patients
Author(s) -
Okada Noriko,
Wu Xiaoshan,
Mizokami Masashi,
Irie Reiko F.,
Okada Hidechika
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02462.x
Subject(s) - biology , monoclonal antibody , cytolysis , ex vivo , virology , antibody , ganglioside , immunology , in vivo , virus , monoclonal , microbiology and biotechnology , in vitro , cytotoxicity , biochemistry
HIV‐1 infection induces aberrant ganglioside GM2 expression on infected cell lines, and human IgM anti‐GM2 monoclonal antibody (L55 Ab) together with normal fresh human serum (FHS) as a source of complement causes complement mediated cytolysis of HIV‐1 infected cells as well as HIV‐1 particles. We report here that high expression of GM2 was also detected on HIV‐1 infected lymphocytes from HIV‐1 seropositive patients. L55 Ab effectively suppressed the generation of HIV in the presence of FHS in primarily cultured lymphocytes from HIV‐1 infected patients in ex vivo experiments, and the suppression was enhanced additively by AZT. These data suggest that L55 Ab may increase the therapeutic effect of chemotherapy.