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Phenotypic and Genotypic Homogeneity of the Strains of Rickettsia japonica Isolated from Patients with Oriental Spotted Fever
Author(s) -
Uchiyama Tsuneo
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02461.x
Subject(s) - biology , antigenicity , spotted fever , genotype , rickettsiaceae , rickettsia , microbiology and biotechnology , polymerase chain reaction , restriction enzyme , gene , restriction fragment length polymorphism , phenotype , virology , antigen , genetics , virus
Nine pathogenic strains of Rickettsia japonica isolated from patients with Oriental spotted fever were compared phenotypically and genotypically. Constitution and antigenicity of the proteins demonstrated to be the same among strains. Polymerase chain reaction (PCR) amplification of the two major outer membrane protein genes ( ompA and ompB ) and an intracellular spotted fever group‐common antigen protein gene ( rps120 ) produced the same sizes of products for all strains. Restriction fragment length polymorphism of the PCR products showed the same pattern among strains with each endonuclease. Thus, these strains belong to a single type, the same as the type strain YH (=ATCC VR‐1363).