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Salt‐Sensitive Growth of Staphylococcus aureus: Stimulation of Salt‐Induced Autolysis by Multiple Environmental Factors
Author(s) -
Ochiai Toshiro
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02459.x
Subject(s) - autolysis (biology) , autolysin , salt (chemistry) , staphylococcus aureus , biology , growth medium , microbiology and biotechnology , ionic strength , bacterial growth , stimulation , food science , biochemistry , biophysics , bacteria , chemistry , endocrinology , enzyme , antibiotics , genetics , aqueous solution , streptococcus pneumoniae
The growth of Staphylococcus aureus 209P became extremely sensitive to a high NaCl concentration following lowered temperature, reduced air‐supply, and decreased Ca 2+ concentration in the medium. Cells in high‐NaCl and low‐Ca 2 concentration media either autolyzed or transformed into protoplast‐like forms during growth when grown standing below 37 C. The abnormal growth, however, was invariably avoided by preliminary supplementation with polyanetholesulfonate (autolysin inhibitor) in the growth media. These results suggested that the autolytic activity of this organism was precisely controlled by multiple environmental factors such as ionic strength, temperature, air supply, and the concentration of Ca 2+ .